Catalyzes the formation of phosphodiester bonds between adjacent DNA fragments
T4 DNA Ligase facilitates the connection of two cohesive- or blunt-ended DNA strands, linking the 5'-phosphate and 3'-hydroxyl groups of neighboring nucleotides. It is commonly used in molecular biology to create recombinant DNA molecules by ligating a piece of foreign DNA into a vector or to repair damaged DNA by sealing nicks or gaps in the DNA backbone.
Our T4 DNA Ligase can be customized to fit your needs and is available in lyophilization-ready and lyophilized formats.
Applications
- Cloning
- Gene synthesis
- Site-directed mutagenesis
- NGS library preparation
Fortis’s T4 ligase was incubated with overhanging double-stranded DNA oligos and double-stranded truncated adapters at 20C for 20mins. Fortis T4 DNA Ligase demonstrates equivalent ligation efficiency as compared to competitor
Our GMP manufacturing sites are ISO 13485:2016 Certified and FDA-registered.