InhibiTaqTM Master Mix
Inhibitor-tolerant PCR Master Mix
Inhibitors can be a major challenge in nucleic acid amplification, particularly in PCR and qPCR assays, where even trace amounts of inhibitors can compromise the sensitivity and accuracy of the results.
InhibiTaqTM from Fortis Life Sciences is an advanced PCR master mix resistant to a broad range of common PCR inhibitors. InhibiTaqTM Master Mix is compatible with endpoint and real-time assays and can be used in conjunction with RTScript reverse transcriptase to detect RNA and DNA. This helps improve the accuracy and sensitivity of nucleic acid amplification reactions.
Available in lyophilization-ready and lyophilized formats
Figure 1: Inhibitor titration for determining maximum tolerated concentration. A two-fold serial dilution of melanin (starting at 350 μg/mL) was applied to real-time qPCR reactions for the human RNaseP (RPP30) gene using 25 ng of purified human genomic DNA as a detection template.
Features & Benefits
- Compatible with endpoint, real-time assays, and multiplex PCR
- Can be used in conjunction with RTScript reverse transcriptase to detect RNA as well as DNA
- Can be utilized for detecting nucleic acids directly from biological samples without prior extraction
- Available in custom formats to fit your needs!
*Our GMP manufacturing sites are ISO 13485:2016 Certified and FDA-registered
| Inhibitor | Standard Taq polymerase | InhibiTaq |
|---|---|---|
| Hematin (μg/mL) | 21.8 | 87.5 |
| Urea (mg/mL) | 8.9 | 17.5 |
| Collagen (μg/mL) | 43.8 | 87.5 |
| Melanin (μg/mL) | 2.7 | 21.9 |
| Bile salt (mg/mL) | 0.5 | 1.1 |
