If you are experiencing difficulties with a Bethyl product, please first consult the Frequently Asked Questions below. If your question isn't answered, please complete the form as accurately as possible. The information is helpful to troubleshoot the differences between the observed results and the expected results. Once submitted, a Technical Support representative will contact you within 1 business day. Please note that all comments and suggestions are taken seriously by Bethyl Laboratories.
IP Support FAQs
When probing immunoprecipitated samples on a western blot, the concentration of primary antibody can be increased resulting in an increase in sensitivity. However, for best results, the optimal dilution of antibody should be empirically determined.
Inhibitors are not necessary for the wash the steps.
For best results, the optimal amounts of antibody should be empirically determined. But a general rule is to add 2 to 10 micrograms of antibody per 500 micrograms of lysate. If you are using neat antisera, or an IgG fraction (such as protein-A purified antibody), greater amounts of antibody are likely to be required.
Protein A/G cross-reactivity is minimized by the addition of pig serum to the blocking buffer. Heavy- and light-chain cross-reactivity is minimized by using an anti-IgG light chain secondary antibody (A120-113P) for detection.
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