Isothermal DNA Amplification for Molecular Diagnostics

Isothermal amplification is a method that enables nucleic acid detection at a fixed temperature, with this feature making isothermal amplification desirable for use in rapid, portable, point-of-care (POC) assays.

There are several different isothermal amplification methods which utilize different enzymes and master mix components for different applications. For example, Loop-mediated isothermal amplification (LAMP) requires Bst polymerase and works at 65 °C to increase specificity; whereas, Whole Genome Amplification (WGA) requires Phi29 Polymerase and works at lower temperatures (30±5°C). All isothermal amplification methods require DNA polymerases with high strand displacement activity, sequence specific primers for specificity to the target gene, single stranded binding proteins, and secondary enzymes.

At Fortis, we develop and manufacture custom enzymes, proteins, and master mixes that meet all your needs for isothermal amplification assay development - whether it be for detecting RNA or DNA from human, animal or environmental samples on a lateral flow strip or using another detection method.