Rabbit anti-CD28 Antibody Affinity Purified
Product Details
Specifications
The epitope recognized by A304-418A maps to a region between residue 1 to 50 of human CD28 Molecule using the numbering given in entry NP_006130.1 (GeneID 940).
Immunoglobulin concentration was determined using Beer’s Law where 1mg/mL IgG has an A280 of 1.4. Antibody was affinity purified using an epitope specific to CD28 immobilized on solid support.
The epitope recognized by A304-418A-T maps to a region between residue 1 to 50 of human CD28 Molecule using the numbering given in entry NP_006130.1 (GeneID 940).
Additional Product Information
CD28 is involved in T-cell activation, the induction of cell proliferation and cytokine production, and promotion of T-cell survival [taken from the Universal Protein Resource (UniProt) www.uniprot.org/uniprot/P10747].
Alternate Names
CD antigen CD28; CD28 antigen; T-cell-specific surface glycoprotein CD28; Tp44
Applications
All western blot analysis is performed using 5% Milk-TBST for blocking and as antibody diluent. Primary antibody is incubated overnight.
Western blots of cell lysates are performed using Goat anti-Rabbit IgG Heavy and Light Chain Antibody (Cat. No. A120-101P).
Western blots of immunoprecipitates are performed using Goat anti-Rabbit Light Chain HRP Conjugate (Cat. No. A120-113P) with 5% Normal Pig Serum (Cat. No. S100-020) added to the blocking buffer.