Rabbit anti-CEACAM1/5 Recombinant Monoclonal Antibody [BLR032F]
Product Details
Specifications
Request Formulation Change Borate Buffered Saline (BBS) pH 8.2 with 0.09% Sodium Azide, BSA-Free
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Immunogen was a recombinant protein representing the extracellular domain of human CEACAM1 (residues 35-433, NP_001020083.1).
By western blot of transfected cells expressing CEACAM1 and transfected cells expressing CEACAM5, this antibody was confirmed to bind both CEACAM1 and CEACAM5.
Additional Product Information
Carcinoembryonic antigen related cell adhesion molecules 1 and 5 (CEACAM1 and CEACAM5) are members of the carcinoembryonic antigen (CEA) gene family, which belongs to the immunoglobulin superfamily. CEACAM1 was originally described in bile ducts of liver as biliary glycoprotein. Subsequently, it was found to be a cell-cell adhesion molecule detected on leukocytes, epithelia, and endothelia. CEACAM5 is used as a clinical biomarker for gastrointestinal cancers and may promote tumor development through its role as a cell adhesion molecule. Additionally, CEACAM5 may regulate differentiation, apoptosis, and cell polarity.
Alternate Names
antigen CD66; BGP; BGP1; BGP-1; BGPI; Biliary glycoprotein 1; carcinoembryonic antigen-related cell adhesion molecule 1; carcinoembryonic antigen-related cell adhesion molecule 1 (biliary glycoprotein); CD antigen CD66a; CD66a antigen
Applications
All western blot analysis is performed using 5% Milk-TBST for blocking and as antibody diluent. Primary antibody is incubated overnight.
Western blots of cell lysates are performed using Goat anti-Rabbit IgG Heavy and Light Chain Antibody (Cat. No. A120-101P).
Western blots of immunoprecipitates are performed using Goat anti-Rabbit Light Chain HRP Conjugate (Cat. No. A120-113P) with 5% Normal Pig Serum (Cat. No. S100-020) added to the blocking buffer.