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Rabbit anti-HSP60 Antibody Affinity Purified

bethyl
Bethyl Laboratories Catalog #
Validated
Target:
HSP60
Reactivity:
Human, Mouse
Host:
Rabbit
Clonality:
Polyclonal
Format:
Whole IgG
Immunogen:
Between 250 and 300
Isotype:
IgG
Conjugate:
Unconjugated
Purity:
Antigen Affinity Purified
100 µl (200 µg/ml)

10 µl (200 µg/ml)

$131.00 $464.00
Qty:

Product Details

Specifications

Verified Reactivity
Mouse, Human
Presumed Reactivity
Golden Hamster, Bovine, Rat
Antigen Species
Human
Concentration
200 µg/ml
Storage
2 - 8 °C
Shelf Life
1 year from date of receipt
Physical State
Liquid
Buffer
Tris-buffered Saline containing 0.1% BSA and 0.09% Sodium Azide
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Production Procedures
Antibody was affinity purified using an epitope specific to HSP60 immobilized on solid support.

The epitope recognized by A302-845A maps to a region between residue 250 and 300 of human Heat Shock Protein 60 using the numbering given in entry NP_002147.2 (GeneID 3329).

Immunoglobulin concentration was determined using Beer’s Law where 1mg/mL IgG has an A280 of 1.4.
Antibody was affinity purified using an epitope specific to HSP60 immobilized on solid support.

The epitope recognized by A302-845A-T maps to a region between residue 250 and 300 of human Heat Shock Protein 60 using the numbering given in entry NP_002147.2 (GeneID 3329).
Country of Origin
USA

Additional Product Information

Heat shock protein 60 (HSP60) is a member of the chaperonin family. HSP60 is a mitochondrial protein that may function as a signaling molecule in the innate immune system. It is essential for the folding and assembly of newly imported proteins in the mitochondria. [taken from NCBI Entrez Gene (GeneID: 3329)].

3329
HSPD1
heat shock protein family D (Hsp60) member 1
P10809
60 kDa heat shock protein, mitochondrial

Alternate Names

60 kDa chaperonin; 60 kDa heat shock protein, mitochondrial; chaperonin 60; CPN60; epididymis secretory sperm binding protein; GROEL; heat shock 60kDa protein 1 (chaperonin); Heat shock protein 60; heat shock protein 65; HLD4; HSP60; HSP-60; HSP65; HuCHA60; mitochondrial matrix protein P1; P60 lymphocyte protein; short heat shock protein 60 Hsp60s1; SPG13

Applications

All western blot analysis is performed using 5% Milk-TBST for blocking and as antibody diluent. Primary antibody is incubated overnight.

Western blots of cell lysates are performed using Goat anti-Rabbit IgG Heavy and Light Chain Antibody (Cat. No. A120-101P).

Western blots of immunoprecipitates are performed using Goat anti-Rabbit Light Chain HRP Conjugate (Cat. No. A120-113P) with 5% Normal Pig Serum (Cat. No. S100-020) added to the blocking buffer.