Rabbit anti-Catalase/CAT Antibody Affinity Purified
Product Details
Specifications
The epitope recognized by A304-767A maps to a region between residue 477 to 527 of human Catalase using the numbering given in entry P04040 (GeneID 847).
Immunoglobulin concentration was determined using Beer’s Law where 1mg/mL IgG has an A280 of 1.4. Antibody was affinity purified using an epitope specific to Catalase/CAT immobilized on solid support.
The epitope recognized by A304-767A-T maps to a region between residue 477 to 527 of human Catalase using the numbering given in entry P04040 (GeneID 847).
Additional Product Information
CAT is a catalase, a key antioxidant enzyme in the bodies defense against oxidative stress. Catalase is a heme enzyme that is present in the peroxisome of nearly all aerobic cells. Catalase converts the reactive oxygen species hydrogen peroxide to water and oxygen and thereby mitigates the toxic effects of hydrogen peroxide. Oxidative stress is hypothesized to play a role in the development of many chronic or late-onset diseases such as diabetes, asthma, Alzheimer's disease, systemic lupus erythematosus, rheumatoid arthritis, and cancers [taken from NCBI Entrez Gene (Gene ID: 847)].
Alternate Names
catalase; epididymis secretory sperm binding protein
Applications
All western blot analysis is performed using 5% Milk-TBST for blocking and as antibody diluent. Primary antibody is incubated overnight.
Western blots of cell lysates are performed using Goat anti-Rabbit IgG Heavy and Light Chain Antibody (Cat. No. A120-101P).
Western blots of immunoprecipitates are performed using Goat anti-Rabbit Light Chain HRP Conjugate (Cat. No. A120-113P) with 5% Normal Pig Serum (Cat. No. S100-020) added to the blocking buffer.