Rabbit anti-SRP9 Antibody Affinity Purified
Product Details
Specifications
The epitope recognized by A305-455A maps to a region between residue 36 to 86 of human Signal recognition particle 9 kDa protein using the numbering given in entry P49458.2 (GeneID 6726).
Immunoglobulin concentration was determined using Beer’s Law where 1mg/mL IgG has an A280 of 1.4. Antibody was affinity purified using an epitope specific to SRP9 immobilized on solid support.
The epitope recognized by A305-455A-T maps to a region between residue 36 to 86 of human Signal recognition particle 9 kDa protein using the numbering given in entry P49458.2 (GeneID 6726).
Additional Product Information
Signal-recognition-particle assembly has a crucial role in targeting secretory proteins to the rough endoplasmic reticulum membrane. Signal recognition particle 9 kDa protein (SRP9) together with SRP14 and the Alu portion of the SRP RNA, constitutes the elongation arrest domain of SRP. The complex of SRP9 and SRP14 is required for SRP RNA binding [taken from the Universal Protein Resource (UniProt) www.uniprot.org/uniprot/P49458].
Alternate Names
ALURBP; signal recognition particle 9 kDa protein; signal recognition particle 9kD; signal recognition particle 9kDa; SRP9
Applications
All western blot analysis is performed using 5% Milk-TBST for blocking and as antibody diluent. Primary antibody is incubated overnight.
Western blots of cell lysates are performed using Goat anti-Rabbit IgG Heavy and Light Chain Antibody (Cat. No. A120-101P).
Western blots of immunoprecipitates are performed using Goat anti-Rabbit Light Chain HRP Conjugate (Cat. No. A120-113P) with 5% Normal Pig Serum (Cat. No. S100-020) added to the blocking buffer.