Purified Human IgG-Fc Fragment normal serum
Product Details
Specifications
Protein concentration was determined using Beer’s Law where 1mg/mL IgG has an A280 of 1.4.
By immunoelectrophoresis the IgG was shown to 1) react with antiserum specific for human IgG-Fc, 2) not react with antiserum specific for IgA, IgM, kappa or lambda chain and 3) produce a single precipitin arc with antiserum against human serum identical to that produced with anti-IgG-Fc.
By ELISA the IgG contains less than 1% of the other immunoglobulin classes and light chains kappa and lambda.
Additional Product Information
IgG is the primary immunoglobulin protein found in blood/plasma, and functions to neutralize toxins, active complement, immobilizing pathogens and inducing opsonization, and cell-mediated cytotoxicity. The anti-Fc activity ensures activity only to the Fc portion of the IgG molecule and not the Fab fragments on the light chain.
Applications
Used as a positive control in immunoassays.
Serum or plasma from donor has been tested and found negative for HIV-1 antigen, antibodies for HIV and HCV, and non-reactive for HBsAg, HIV-1 RNA, and HCV RNA. Not tested for AntiHBc. HANDLE AS IF CAPABLE OF TRANSMITTING INFECTIOUS AGENTS.
Used as a positive control in immunoassays.
Serum or plasma from donor has been tested and found negative for HIV-1 antigen, antibodies for HIV and HCV, and non-reactive for HBsAg, HIV-1 RNA, and HCV RNA. Not tested for AntiHBc. HANDLE AS IF CAPABLE OF TRANSMITTING INFECTIOUS AGENTS.