Goat anti-Human Hemoglobin Antibody Biotinylated
Product Details
Specifications
Request Formulation Change Phosphate Buffered Saline (PBS) containing 0.2% BSA and 0.05% Pro-Clean 400
Request Formulation Change Phosphate Buffered Saline (PBS) containing 0.09% Sodium Azide
Request Formulation Change Serum containing 0.09% Sodium Azide
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Immunoglobulin concentration was determined using Beer’s Law where 1mg/mL IgG has an A280 of 1.4.
By immunoelectrophoresis and ELISA this antibody reacts specifically with human hemoglobin A1, A2, F and S. There is no reaction with human serum proteins Goats were immunized with purified human hemoglobin A1 of adult red blood cell origin. The antibody was isolated by affinity chromatography using antigen coupled to agarose beads and conjugated to horseradish peroxidase (HRP).
Prior to conjugation, immunoglobulin concentration was determined using Beer’s Law where 1mg/mL IgG has an A280 of 1.4. Molar enzyme/antibody protein ratio is 4:1.
By immunoelectrophoresis and ELISA this antibody reacts specifically with human hemoglobin A1, A2, F and S. There is no reaction with human serum proteins. Goats were immunized with purified human hemoglobin A1 of adult red blood cell origin. The antibody was isolated by affinity chromatography using antigen coupled to agarose beads. Immunoglobulin concentration was determined using Beer’s Law where 1mg/mL IgG has an A280 of 1.4.
By immunoelectrophoresis and ELISA this antibody reacts specifically with human hemoglobin A1, A2, F and S. There is no reaction with human serum proteins Goats were immunized with purified human hemoglobin A1 of adult red blood cell origin. The antibody was isolated by affinity chromatography using antigen coupled to agarose beads and conjugated to fluorescein isothiocyanate (FITC).
Prior to conjugation, immunoglobulin concentration was determined using Beer’s Law where 1mg/mL IgG has an A280 of 1.4.
By immunoelectrophoresis and ELISA this antibody reacts specifically with human hemoglobin A1, A2, F and S. There is no reaction with human serum proteins. Goats were immunized with purified human hemoglobin A1 of adult red blood cell origin. By immunoelectrophoresis, the antiserum reacts specifically with human hemoglobin A1, A2, F and S. No antibody was detected against human serum proteins.
Additional Product Information
Hemoglobin is tetrameric protein that binds iron in individual heme groups. These molecules are responsible for the binding and transport of oxygen in the blood and are the most abundant component of red blood cells.
Applications
Not all listed applications have been specifically tested by our laboratory.
For use in precipitin gel reactions (e.g. immunoelectrophoresis (IEP) or double diffusion Ouchterlony (DD)).
Optimal working dilutions should be determined experimentally by the investigator.