Goat anti-RFC4 Antibody Affinity Purified
Product Details
Specifications
The epitope recognized by A300-144A-T maps to a region between residues 1 and 50 of human Replication Factor C subunit 4 using the numbering given in entry NP_002907.1 (GeneID 5984).
Immunoglobulin concentration was determined using Beer’s Law where 1mg/mL IgG has an A280 of 1.4. Antibody was affinity purified using an epitope specific to RFC4 immobilized on solid support.
The epitope recognized by A300-144A maps to a region between residues 1 and 50 of human Replication Factor C subunit 4 using the numbering given in entry NP_002907.1 (GeneID 5984).
Immunoglobulin concentration was determined using Beer’s Law where 1mg/mL IgG has an A280 of 1.4.
Additional Product Information
The Replication factor C (RFC) heteropentamer includes RFC1, RFC2, RFC3, RFC4, and RFC5. The RFC heteropentamer is part of the multiprotein DNA replicase that functions to replicate DNA prior to cell division and repair DNA damage. RFC is the clamp loader ATPase associated with the DNA replicase. As the clamp loader, RFC uses ATP to recruit, open, and close the PCNA ring to link it to DNA for replication and repair. RFC4 is the 37 kDa subunit of RFC that is part of the core complex formed with RFC2 and RFC5.
Alternate Names
A1; A1 37 kDa subunit; activator 1 37 kDa subunit; activator 1 subunit 4; replication factor C (activator 1) 4, 37kDa; replication factor C 37 kDa subunit; replication factor C subunit 4; RFC 37 kDa subunit; RF-C 37 kDa subunit; RFC37
Applications
All western blot analysis is performed using 5% Milk-TBST for blocking and as antibody diluent. Primary antibody is incubated overnight.
Western blots are performed using Donkey anti-Goat IgG Heavy and Light Chain Antibody (Cat. No. A50-101P).