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Mouse anti-CD45RO Monoclonal Antibody Purified [UCHL-1]

bethyl
Bethyl Laboratories Catalog #
Validated
Target:
CD45RO
Reactivity:
Human
Platforms:
COMET™, PhenoImager™ HT
Host:
Mouse
Clonality:
Monoclonal
Clone:
UCHL-1
Format:
Whole IgG
Immunogen:
Cultured T-cells from an IL-2-dependent T-cell line (CA1)
Isotype:
IgG2a
Conjugate:
Unconjugated
Purity:
Purified
100 µl (50+ tests)

10 µl (5+ tests)

100 µg (BSA-Free)

$127.00 $691.00
Qty:

Product Details

Specifications

Verified Reactivity
Human
Antigen Species
Human
Concentration
50 µg/ml 1000 µg/ml
Storage
2 - 8 °C
Shelf Life
1 year from date of receipt
Physical State
Liquid
Buffer
Phosphate Buffered Saline (PBS) containing 0.09% Sodium Azide
Request Formulation Change Phosphate Buffered Saline (PBS) containing 0.09% Sodium Azide, BSA-Free
Request Formulation Change
Production Procedures
The antibody was purified from cell culture supernatant.

Immunogen: Cultured T-cells from an IL-2-dependent T-cell line (CA1).
Country of Origin
USA

Additional Product Information

CD45 is a protein tyrosine-protein phosphatase required for T-cell activation through the antigen receptor. CD45 acts as a positive regulator of T-cell coactivation upon binding to DPP4. The first PTPase domain has enzymatic activity, while the second one seems to affect the substrate specificity of the first one. Upon T-cell activation, CD45 recruits and dephosphorylates SKAP1 and FYN [taken from the Universal Protein Resource (UniProt) www.uniprot.org/uniprot/P08575].

5788
PTPRC
protein tyrosine phosphatase, receptor type C
P08575
Receptor-type tyrosine-protein phosphatase C

Alternate Names

B220; CD antigen CD45; CD45; CD45 antigen; CD45R; GP180; LCA; L-CA; Leukocyte common antigen; LY5; protein tyrosine phosphatase, receptor type, c polypeptide; receptor-type tyrosine-protein phosphatase C; T200; T200 glycoprotein; T200 leukocyte common antigen

Applications

All western blot analysis is performed using 5% Milk-TBST for blocking and as antibody diluent. Primary antibody is incubated overnight.

Western blots are performed using Goat anti-Mouse IgG Heavy and Light Chain Antibody (Cat. No. A90-216P).