Mouse anti-PCNA Monoclonal Antibody Purified [PC10]
Product Details
Specifications
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Immunogen was recombinant rat PCNA Monoclonal antibody was purified from cell culture supernatant.
Immunogen was recombinant rat PCNA.
Additional Product Information
PCNA (Proliferating Cell Nuclear Antigen) was first identified as a factor expressed in the nuclei of replicating cells. PCNA plays a role in both DNA synthesis and repair. PCNA functions as a processivity factor for DNA polymerase by acting as a DNA clamp that tethers the polymerase catalytic units to the DNA template. The structure of PCNA is that of a trimeric ring that encircles and slides along replicating DNA. PCNA interacts with and is regulated by several proteins involved in cell cycle control and check point processes.
Alternate Names
ATLD2; cyclin; DNA polymerase delta auxiliary protein; PCNA; proliferating cell nuclear antigen
Applications
All western blot analysis is performed using 5% Milk-TBST for blocking and as antibody diluent. Primary antibody is incubated overnight.
Western blots are performed using Goat anti-Mouse IgG Heavy and Light Chain Antibody (Cat. No. A90-216P).