Rabbit anti-4E-T/eIF4E-T Antibody Affinity Purified
Product Details
Specifications
The epitope recognized by A300-706A maps to a region between residue 925 and the C-terminus (residue 985) of human Eukaryotic Translation Initiation Factor 4E Nuclear Import Factor 1 using the numbering given in entry NP_062817.1 (GeneID 56478).
Immunoglobulin concentration was determined using Beer’s Law where 1mg/mL IgG has an A280 of 1.4. Antibody was affinity purified using an epitope specific to 4E-t/eIF4E-T immobilized on solid support.
The epitope recognized by A300-706A-T maps to a region between residue 925 and the C-terminus (residue 985) of human Eukaryotic Translation Initiation Factor 4E Nuclear Import Factor 1 using the numbering given in entry NP_062817.1 (GeneID 56478).
Additional Product Information
Eukaryotic translation initiation factor 4E nuclear import factor 1 (4ET) functions as a nucleoplasmic shuttle protein that mediates nuclear import of the translation initiation factor eIF4E.
Alternate Names
2610509L04Rik; 4E-T; Clast4; eIF4E transporter; Eukaryotic translation initiation factor 4E nuclear import factor 1; eukaryotic translation initiation factor 4E transporter
Applications
All western blot analysis is performed using 5% Milk-TBST for blocking and as antibody diluent. Primary antibody is incubated overnight.
Western blots of cell lysates are performed using Goat anti-Rabbit IgG Heavy and Light Chain Antibody (Cat. No. A120-101P).
Western blots of immunoprecipitates are performed using Goat anti-Rabbit Light Chain HRP Conjugate (Cat. No. A120-113P) with 5% Normal Pig Serum (Cat. No. S100-020) added to the blocking buffer.