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Rabbit anti-6-His Tag Antibody Affinity Purified
Bethyl Laboratories
Catalog #
Target:
6-His Tag
Applications:
ELISA
,ICC
,IF
,IP
,WB
Host:
Rabbit
Clonality:
Polyclonal
Format:
Whole IgG
Isotype:
IgG
Conjugate:
Biotin
,FITC
,HRP
,Unconjugated
Purity:
Antigen Affinity Purified
Product Details
Specifications
Concentration
1 mg/ml
Storage
2 - 8 °C
Shelf Life
1 year from date of receipt
Physical State
Liquid
Buffer
Phosphate Buffered Saline (PBS) containing 0.09% Sodium Azide
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Phosphate Buffered Saline (PBS) containing 0.2% BSA and 0.05% Pro-Clean 400
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Phosphate Buffered Saline (PBS) containing 0.2% BSA and 0.09% Sodium Azide
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Production Procedures
Rabbits were immunized with 6-His (HHHHHH) conjugated to KLH. Antibody was isolated by affinity chromatography using the peptide immobilized on solid support. This antibody does not detect all N-terminal 6-His tagged proteins.
Immunoglobulin concentration was determined using Beer’s Law where 1mg/mL IgG has an A280 of 1.4.
Rabbits were immunized with 6-His (HHHHHH) conjugated to KLH. Antibody was isolated by affinity chromatography using the peptide immobilized on solid support and conjugated to horseradish peroxidase (HRP). This antibody does not detect all N-terminal 6-His tagged proteins.
Prior to conjugation, immunoglobulin concentration was determined using Beer’s Law where 1mg/mL IgG has an A280 of 1.4. Molar enzyme/antibody protein ratio is 4:1.
Rabbits were immunized with 6-His (HHHHHH) conjugated to KLH. Antibody was isolated by affinity chromatography using the peptide immobilized on solid support and conjugated to fluorescein isothiocyanate (FITC). This antibody does not detect all N-terminal 6-His tagged proteins.
Prior to conjugation, immunoglobulin concentration was determined using Beer’s Law where 1mg/mL IgG has an A280 of 1.4.
Rabbits were immunized with 6-His (HHHHHH) conjugated to KLH. Antibody was isolated by affinity chromatography using the peptide immobilized on solid support and conjugated to biotin. This antibody does not detect all N-terminal 6-His tagged proteins.
Prior to conjugation, immunoglobulin concentration was determined using Beer’s Law where 1mg/mL IgG has an A280 of 1.4.
Country of Origin
USA
Additional Product Information
The 6-His tag is a commonly used epitope tag that can be added to a recombinantly expressed protein for the purposes of protein purification or detection of protein. Typically utilizes metal affinity chromatography for purification and elutes well imidazole.
Applications