Rabbit anti-AAK1 Antibody Affinity Purified
Product Details
Specifications
The epitope recognized by A302-146A maps to a region between residue 350 and 410 of human AP2 associated kinase 1 using the numbering given in entry AAI11966.1 (GeneID 22848).
Immunoglobulin concentration was determined using Beer’s Law where 1mg/mL IgG has an A280 of 1.4. Antibody was affinity purified using an epitope specific to AAK1 immobilized on solid support.
The epitope recognized by A302-146A-T maps to a region between residue 350 and 410 of human AP2 associated kinase 1 using the numbering given in entry AAI11966.1 (GeneID 22848).
Additional Product Information
AAK1 (AP2-associated kinase 1) is a Ser/Thr protein kinase. The rat homolog was first identified in a screen of a rat brain cDNA library using a domain of the alpha subunit of the heterotetrameric AP2 complex as bait. The AP2 complex is one of two major coat proteins of endocytic vesicles that function in receptor-mediated endocytosis and the recycling of synaptic vesicles. AAK1 is responsible for phosphorylating the mu subunit of AP2 to promote clathrin-mediated endocytosis.
Alternate Names
adaptor-associated kinase 1; AP2-associated protein kinase 1
Applications
All western blot analysis is performed using 5% Milk-TBST for blocking and as antibody diluent. Primary antibody is incubated overnight.
Western blots of cell lysates are performed using Goat anti-Rabbit IgG Heavy and Light Chain Antibody (Cat. No. A120-101P).
Western blots of immunoprecipitates are performed using Goat anti-Rabbit Light Chain HRP Conjugate (Cat. No. A120-113P) with 5% Normal Pig Serum (Cat. No. S100-020) added to the blocking buffer.