Rabbit anti-AARS Antibody Affinity Purified
Product Details
Specifications
The epitope recognized by A303-475A maps to a region between residue 918 and 968 of human Alanyl-tRNA Synthetase using the numbering given in entry NP_001596.2 (GeneID 16).
Immunoglobulin concentration was determined using Beer’s Law where 1mg/mL IgG has an A280 of 1.4. Antibody was affinity purified using an epitope specific to AARS immobilized on solid support.
The epitope recognized by A303-475A-T maps to a region between residue 918 and 968 of human Alanyl-tRNA Synthetase using the numbering given in entry NP_001596.2 (GeneID 16).
Additional Product Information
Alanyl-tRNA synthetase, cytoplasmic (AARS) catalyzes the attachment of alanine to tRNA(Ala) in a two-step reaction: alanine is first activated by ATP to form Ala-AMP and then transferred to the acceptor end of tRNA(Ala). AARS also edits incorrectly charged tRNA(Ala) via its editing domain [taken from the Universal Protein Resource (UniProt) www.uniprot.org/uniprot/P49588].
Alternate Names
alanine tRNA ligase 1, cytoplasmic; alanine--tRNA ligase, cytoplasmic; Alanyl-tRNA synthetase; alanyl-tRNA synthetase, cytoplasmic; alaRS; CMT2N; EIEE29; renal carcinoma antigen NY-REN-42
Applications
All western blot analysis is performed using 5% Milk-TBST for blocking and as antibody diluent. Primary antibody is incubated overnight.
Western blots of cell lysates are performed using Goat anti-Rabbit IgG Heavy and Light Chain Antibody (Cat. No. A120-101P).
Western blots of immunoprecipitates are performed using Goat anti-Rabbit Light Chain HRP Conjugate (Cat. No. A120-113P) with 5% Normal Pig Serum (Cat. No. S100-020) added to the blocking buffer.