Rabbit anti-ADAR1 Antibody Affinity Purified
Product Details
Specifications
The epitope recognized by A303-883A maps to a region between residue 200 and 250 of human Adenosine Deaminase, RNA-specific using the numbering given in entry NP_001102.2 (GeneID 103).
Immunoglobulin concentration was determined using Beer’s Law where 1mg/mL IgG has an A280 of 1.4. Antibody was affinity purified using an epitope specific to ADAR1 immobilized on solid support.
The epitope recognized by A303-883A-T maps to a region between residue 200 and 250 of human Adenosine Deaminase, RNA-specific using the numbering given in entry NP_001102.2 (GeneID 103).
Additional Product Information
ADAR1 (adenosine deaminase, RNA specific) is an enzyme responsible for RNA editing by site-specific deamination of adenosines. This enzyme destabilizes double-stranded RNA through conversion of adenosine to inosine. Mutations in this gene have been associated with dyschromatosis symmetrica hereditary [taken from NCBI Entrez Gene (Gene ID: 103)].
Alternate Names
136 kDa double-stranded RNA-binding protein; ADAR1; adenosine deaminase acting on RNA 1-A; AGS6; double-stranded RNA-specific adenosine deaminase; DRADA; DSH; DSRAD; dsRNA adenosine deaminase; dsRNA adeonosine deaminase; G1P1; IFI4; IFI-4; interferon-induced protein 4; interferon-inducible protein 4; K88DSRBP; P136
Applications
All western blot analysis is performed using 5% Milk-TBST for blocking and as antibody diluent. Primary antibody is incubated overnight.
Western blots of cell lysates are performed using Goat anti-Rabbit IgG Heavy and Light Chain Antibody (Cat. No. A120-101P).
Western blots of immunoprecipitates are performed using Goat anti-Rabbit Light Chain HRP Conjugate (Cat. No. A120-113P) with 5% Normal Pig Serum (Cat. No. S100-020) added to the blocking buffer.