Rabbit anti-ADK Antibody Affinity Purified
Product Details
Specifications
The epitope recognized by A304-280A maps to a region between residue 312 to 362 of human Adenosine Kinase using the numbering given in entry NP_006712.2 (GeneID 132).
Immunoglobulin concentration was determined using Beer’s Law where 1mg/mL IgG has an A280 of 1.4. Antibody was affinity purified using an epitope specific to ADK immobilized on solid support.
The epitope recognized by A304-280A-T maps to a region between residue 312 to 362 of human Adenosine Kinase using the numbering given in entry NP_006712.2 (GeneID 132).
Additional Product Information
Adenosine kinase (ADK) is an enzyme which catalyzes the transfer of the gamma-phosphate from ATP to adenosine, thereby serving as a regulator of concentrations of both extracellular adenosine and intracellular adenine nucleotides. Adenosine has widespread effects on the cardiovascular, nervous, respiratory, and immune systems and inhibitors of the enzyme could play an important pharmacological role in increasing intravascular adenosine concentrations and acting as anti-inflammatory agents [taken from NCBI Entrez Gene (Gene ID: 132)].
Alternate Names
adenosine 5'-phosphotransferase; adenosine kinase; AK; testicular tissue protein Li 14
Applications
All western blot analysis is performed using 5% Milk-TBST for blocking and as antibody diluent. Primary antibody is incubated overnight.
Western blots of cell lysates are performed using Goat anti-Rabbit IgG Heavy and Light Chain Antibody (Cat. No. A120-101P).
Western blots of immunoprecipitates are performed using Goat anti-Rabbit Light Chain HRP Conjugate (Cat. No. A120-113P) with 5% Normal Pig Serum (Cat. No. S100-020) added to the blocking buffer.