Rabbit anti-AGTPBP1 Antibody Affinity Purified
Product Details
Specifications
The epitope recognized by A305-295A maps to a region between residue 1176 to 1226 of human Cytosolic carboxypeptidase 1 using the numbering given in entry Q9UPW5.3 (GeneID 23287).
Immunoglobulin concentration was determined using Beer’s Law where 1mg/mL IgG has an A280 of 1.4. Antibody was affinity purified using an epitope specific to AGTPBP1 immobilized on solid support.
The epitope recognized by A305-295A-T maps to a region between residue 1176 to 1226 of human Cytosolic carboxypeptidase 1 using the numbering given in entry Q9UPW5.3 (GeneID 23287).
Additional Product Information
AGTPBP1 is a metallocarboxypeptidase that mediates deglutamylation of target proteins. AGTB1 catalyzes the deglutamylation of polyglutamate side chains generated by post-translational polyglutamylation in proteins such as tubulins [taken from NCBI Entrez Gene (Gene ID: 23287)].
Alternate Names
ATP/GTP-binding protein 1; carboxypeptidase-tubulin; CCP1; cytosolic carboxypeptidase 1; nervous system nuclear protein induced by axotomy protein 1 homolog; NNA1; soluble carboxypeptidase; tubulinyl-Tyr carboxypeptidase; tyrosine carboxypeptidase
Applications
All western blot analysis is performed using 5% Milk-TBST for blocking and as antibody diluent. Primary antibody is incubated overnight.
Western blots of cell lysates are performed using Goat anti-Rabbit IgG Heavy and Light Chain Antibody (Cat. No. A120-101P).
Western blots of immunoprecipitates are performed using Goat anti-Rabbit Light Chain HRP Conjugate (Cat. No. A120-113P) with 5% Normal Pig Serum (Cat. No. S100-020) added to the blocking buffer.