Rabbit anti-AHR Recombinant Monoclonal Antibody [BLR118H]
Product Details
Specifications
Request Formulation Change Borate Buffered Saline (BBS) pH 8.2 with 0.09% Sodium Azide, BSA-Free
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Additional Product Information
AHR is a ligand-activated helix-loop-helix transcription factor involved in the regulation of biological responses to planar aromatic hydrocarbons. AHR has been shown to regulate xenobiotic-metabolizing enzymes such as cytochrome P450. Before ligand binding, AHR is sequestered in the cytoplasm; upon ligand binding, the protein moves to the nucleus and stimulates transcription of target genes. [taken from NCBI Entrez Gene (Gene ID: 196)].
Alternate Names
ah receptor; AhR; AH-receptor; aromatic hydrocarbon receptor; aryl hydrocarbon receptor; bHLHe76; class E basic helix-loop-helix protein 76
Applications
All western blot analysis is performed using 5% Milk-TBST for blocking and as antibody diluent. Primary antibody is incubated overnight.
Western blots of cell lysates are performed using Goat anti-Rabbit IgG Heavy and Light Chain Antibody (Cat. No. A120-101P).
Western blots of immunoprecipitates are performed using Goat anti-Rabbit Light Chain HRP Conjugate (Cat. No. A120-113P) with 5% Normal Pig Serum (Cat. No. S100-020) added to the blocking buffer.