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Rabbit anti-AKAP1 Antibody Affinity Purified

bethyl
Bethyl Laboratories Catalog #
Validated
Target:
AKAP1
Reactivity:
Human
Host:
Rabbit
Clonality:
Polyclonal
Format:
Whole IgG
Immunogen:
Between 450 and 500
Isotype:
IgG
Conjugate:
Unconjugated
Purity:
Antigen Affinity Purified
100 µl (200 µg/ml)

10 µl (200 µg/ml)

$121.00 $433.00
Qty:

Product Details

Specifications

Verified Reactivity
Human
Antigen Species
Human
Concentration
200 µg/ml
Storage
2 - 8 °C
Shelf Life
1 year from date of receipt
Physical State
Liquid
Buffer
Tris-buffered Saline containing 0.1% BSA and 0.09% Sodium Azide
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Production Procedures
Antibody was affinity purified using an epitope specific to AKAP1 immobilized on solid support.

The epitope recognized by A301-380A maps to a region between residue 450 and 500 of human A kinase (PRKA) anchor protein 1 using the numbering given in entry NP_003479.1 (GeneID 8165).

Immunoglobulin concentration was determined using Beer’s Law where 1mg/mL IgG has an A280 of 1.4.
Antibody was affinity purified using an epitope specific to AKAP1 immobilized on solid support.

The epitope recognized by A301-380A-T maps to a region between residue 450 and 500 of human A kinase (PRKA) anchor protein 1 using the numbering given in entry NP_003479.1 (GeneID 8165).
Country of Origin
USA

Additional Product Information

AKAP1 (A-kinase anchor protein 1) was isolated as a protein that interacts with the regulatory subunit of protein kinase A (PKA) as well as the catalytic subunit of protein phosphatase 1 (PPI). AKAP1 plays a role in directing the subcellular localization of PKA to the cytoplasmic face of the outer mitochondrial membrane.

8165
AKAP1
A-kinase anchoring protein 1
Q92667
A-kinase anchor protein 1, mitochondrial

Alternate Names

A kinase (PRKA) anchor protein 1; AKAP; AKAP 149; AKAP121; AKAP149; AKAP84; A-kinase anchor protein 1, mitochondrial; A-kinase anchor protein 149 kDa; D-AKAP1; D-AKAP-1; Dual specificity A-kinase-anchoring protein 1; dual-specificity A-kinase anchoring protein 1; PPP1R43; PRKA1; protein kinase A anchoring protein 1; protein kinase A1; Protein kinase A-anchoring protein 1; protein phosphatase 1, regulatory subunit 43; SAKAP84; S-AKAP84; spermatid A-kinase anchor protein 84; TDRD17; testicular secretory protein Li 5; tudor domain containing 17

Applications

All western blot analysis is performed using 5% Milk-TBST for blocking and as antibody diluent. Primary antibody is incubated overnight.

Western blots of cell lysates are performed using Goat anti-Rabbit IgG Heavy and Light Chain Antibody (Cat. No. A120-101P).

Western blots of immunoprecipitates are performed using Goat anti-Rabbit Light Chain HRP Conjugate (Cat. No. A120-113P) with 5% Normal Pig Serum (Cat. No. S100-020) added to the blocking buffer.