Rabbit anti-AKAP1 Antibody Affinity Purified
Product Details
Specifications
The epitope recognized by A301-380A maps to a region between residue 450 and 500 of human A kinase (PRKA) anchor protein 1 using the numbering given in entry NP_003479.1 (GeneID 8165).
Immunoglobulin concentration was determined using Beer’s Law where 1mg/mL IgG has an A280 of 1.4. Antibody was affinity purified using an epitope specific to AKAP1 immobilized on solid support.
The epitope recognized by A301-380A-T maps to a region between residue 450 and 500 of human A kinase (PRKA) anchor protein 1 using the numbering given in entry NP_003479.1 (GeneID 8165).
Additional Product Information
AKAP1 (A-kinase anchor protein 1) was isolated as a protein that interacts with the regulatory subunit of protein kinase A (PKA) as well as the catalytic subunit of protein phosphatase 1 (PPI). AKAP1 plays a role in directing the subcellular localization of PKA to the cytoplasmic face of the outer mitochondrial membrane.
Alternate Names
A kinase (PRKA) anchor protein 1; AKAP; AKAP 149; AKAP121; AKAP149; AKAP84; A-kinase anchor protein 1, mitochondrial; A-kinase anchor protein 149 kDa; D-AKAP1; D-AKAP-1; Dual specificity A-kinase-anchoring protein 1; dual-specificity A-kinase anchoring protein 1; PPP1R43; PRKA1; protein kinase A anchoring protein 1; protein kinase A1; Protein kinase A-anchoring protein 1; protein phosphatase 1, regulatory subunit 43; SAKAP84; S-AKAP84; spermatid A-kinase anchor protein 84; TDRD17; testicular secretory protein Li 5; tudor domain containing 17
Applications
All western blot analysis is performed using 5% Milk-TBST for blocking and as antibody diluent. Primary antibody is incubated overnight.
Western blots of cell lysates are performed using Goat anti-Rabbit IgG Heavy and Light Chain Antibody (Cat. No. A120-101P).
Western blots of immunoprecipitates are performed using Goat anti-Rabbit Light Chain HRP Conjugate (Cat. No. A120-113P) with 5% Normal Pig Serum (Cat. No. S100-020) added to the blocking buffer.