Rabbit anti-AKAP8/AKAP95 Antibody Affinity Purified
Product Details
Specifications
The epitope recognized by A301-061A maps to a region between residue 575 and 625 of human A-kinase anchor protein 8 using the numbering given in entry NP_005849.1 (GeneID 10270).
Immunoglobulin concentration was determined using Beer’s Law where 1mg/mL IgG has an A280 of 1.4. Antibody was affinity purified using an epitope specific to AKAP8/AKAP95 immobilized on solid support.
The epitope recognized by A301-061A-T maps to a region between residue 575 and 625 of human A-kinase anchor protein 8 using the numbering given in entry NP_005849.1 (GeneID 10270).
Additional Product Information
AKAP8/AKAP95 has been characterized as a 95 kDa protein kinase A (PKA)-anchoring protein and regulatory subunit of PKA that functions to recruit the condensin complex for chromosome condensation during mitosis. A role in DNA replication has also been demonstrated through its interaction with the pre-replication complex component, MCM2 (mini-chromosome maintenance 2). Inhibition of AKAP8/AKAP95 association with MCM2 inhibits replication initiation and elongation in vitro. AKAP8/AKAP95 has also been shown to be involved in apoptosis. KAP8/AKAP95 associates with caspase 3 and serves as a carrier to transport caspase 3 from the cytoplasm to the nucleus to induce morphological changes in the nucleus.
Alternate Names
A kinase (PRKA) anchor protein 8; AKAP 95; AKAP-8; AKAP95; AKAP-95; A-kinase anchor protein 8; A-kinase anchor protein 95 kDa; A-kinase anchor protein, 95kDa
Applications
All western blot analysis is performed using 5% Milk-TBST for blocking and as antibody diluent. Primary antibody is incubated overnight.
Western blots of cell lysates are performed using Goat anti-Rabbit IgG Heavy and Light Chain Antibody (Cat. No. A120-101P).
Western blots of immunoprecipitates are performed using Goat anti-Rabbit Light Chain HRP Conjugate (Cat. No. A120-113P) with 5% Normal Pig Serum (Cat. No. S100-020) added to the blocking buffer.