Rabbit anti-AKT1 IHC Antibody Affinity Purified
Product Details
Specifications
The epitope recognized by IHC-00513 maps to a region between residue 25 and 75 of human v-akt murine thymoma viral oncogene homolog 1 using the numbering given in entry NP_005154.1 (GeneID 207).
Immunoglobulin concentration was determined using Beer’s Law where 1mg/mL IgG has an A280 of 1.4. Antibody was affinity purified using an epitope specific to AKT1 immobilized on solid support.
The epitope recognized by IHC-00513-T maps to a region between residue 25 and 75 of human v-akt murine thymoma viral oncogene homolog 1 using the numbering given in entry NP_005154.1 (GeneID 207).
Immunoglobulin concentration was determined using Beer’s Law where 1mg/mL IgG has an A280 of 1.4.
Additional Product Information
AKT1 is a serine-threonine kinase that functions downstream of PI-3 kinase and is known to phosphorylate several proteins. AKT1 mediates the effects of several growth factors such as insulin, IGF-1, EGF, and PDGF to control cell survival and apoptosis. There are numerous substrates of AKT1, some of which are: GSK3-alpha and beta, mTOR, TSC2, Bad, c-Raf, and caspase 9.
Alternate Names
AKT; AKT1m; CWS6; PKB; PKB alpha; PKB-ALPHA; PRKBA; Protein kinase B; protein kinase B alpha; proto-oncogene c-Akt; RAC; rac protein kinase alpha; RAC-ALPHA; RAC-alpha serine/threonine-protein kinase; RAC-PK-alpha; serine-threonine protein kinase; v-akt murine thymoma viral oncogene homolog 1; v-akt murine thymoma viral oncogene-like protein 1
Applications
Epitope exposure is recommended.
Epitope exposure with citrate buffer will enhance staining.
Likely to work with frozen sections.
In some cases, the antibody may be diluted further than indicated.