Rabbit anti-ALDH18A1/P5CS Antibody Affinity Purified
Product Details
Specifications
The epitope recognized by A304-803A maps to a region between residue 745 to 795 of human Aldehyde Dehydrogenase 18 Family, Member A1 using the numbering given in entry P54886.2 (GeneID 5832).
Immunoglobulin concentration was determined using Beer’s Law where 1mg/mL IgG has an A280 of 1.4. Antibody was affinity purified using an epitope specific to ALDH18A1/P5CS immobilized on solid support.
The epitope recognized by A304-803A-T maps to a region between residue 745 to 795 of human Aldehyde Dehydrogenase 18 Family, Member A1 using the numbering given in entry P54886.2 (GeneID 5832).
Additional Product Information
ALDH18A1/P5CS is a bifunctional enzyme that converts glutamate to glutamate 5-semialdehyde, an intermediate in the biosynthesis of proline, ornithine and arginine [taken from the Universal Protein Resource (UniProt) www.uniprot.org/uniprot/P54886].
Alternate Names
ADCL3; aldehyde dehydrogenase family 18 member A1; ARCL3A; delta1-pyrroline-5-carboxlate synthetase; delta-1-pyrroline-5-carboxylate synthase; delta-1-pyrroline-5-carboxylate synthetase; Gamma-glutamyl kinase; Gamma-glutamyl phosphate reductase (GPR); Glutamate 5-kinase (GK); Glutamate-5-semialdehyde dehydrogenase; Glutamyl-gamma-semialdehyde dehydrogenase; GSAS; P5CS; PYCS; pyrroline-5-carboxylate synthetase (glutamate gamma-semialdehyde synthetase); spastic paraplegia 9 (autosomal dominant); Spastic paraplegia-9 (spastic paraparesis with amyotrophy, cataracts and gastroesophageal reflux); SPG9; SPG9A; SPG9B
Applications
All western blot analysis is performed using 5% Milk-TBST for blocking and as antibody diluent. Primary antibody is incubated overnight.
Western blots of cell lysates are performed using Goat anti-Rabbit IgG Heavy and Light Chain Antibody (Cat. No. A120-101P).
Western blots of immunoprecipitates are performed using Goat anti-Rabbit Light Chain HRP Conjugate (Cat. No. A120-113P) with 5% Normal Pig Serum (Cat. No. S100-020) added to the blocking buffer.