Rabbit anti-alpha-Tubulin Antibody Affinity Purified
Product Details
Specifications
Additional Product Information
alpha-Tubulin is the major constituent of microtubules. It binds two moles of GTP, one at an exchangeable site on the beta chain and one at a non-exchangeable site on the alpha chain. [taken from the Universal Protein Resource (UniProt) www.uniprot.org/uniprot/Q71U36].
Alternate Names
Alpha-tubulin 3; B-ALPHA-1; hum-a-tub1; hum-a-tub2; LIS3; TUBA3; tubulin alpha-1A chain; tubulin alpha-3 chain; tubulin B-alpha-1; tubulin, alpha, brain-specific
Applications
All western blot analysis is performed using 5% Milk-TBST for blocking and as antibody diluent. Primary antibody is incubated overnight.
Western blots of cell lysates are performed using Goat anti-Rabbit IgG Heavy and Light Chain Antibody (Cat. No. A120-101P).
Western blots of immunoprecipitates are performed using Goat anti-Rabbit Light Chain HRP Conjugate (Cat. No. A120-113P) with 5% Normal Pig Serum (Cat. No. S100-020) added to the blocking buffer.