Rabbit anti-AMPK alpha 1 Antibody Affinity Purified
Product Details
Specifications
The epitope recognized by A300-507A maps to a region between residues 350 and 400 of human 5'-AMP-activated Protein Kinase, catalytic Alpha-1 chain using the numbering given in entry BAA36547.1 (GeneID 5562).
Immunoglobulin concentration was determined using Beer’s Law where 1mg/mL IgG has an A280 of 1.4. Antibody was affinity purified using an epitope specific to AMPK alpha 1 immobilized on solid support.
The epitope recognized by A300-507A-T maps to a region between residues 350 and 400 of human 5'-AMP-activated Protein Kinase, catalytic Alpha-1 chain using the numbering given in entry BAA36547.1 (GeneID 5562).
Immunoglobulin concentration was determined using Beer’s Law where 1mg/mL IgG has an A280 of 1.4.
Additional Product Information
AMP-activated protein kinase (AMPK) is a heterotrimeric protein made up of a catalytic alpha subunit, and a regulatory beta and gamma subunit. There are two distinct genes for the alpha subunit, alpha1 and alpha2. AMPK alpha 1 is the serine/threonine kinase catalytic subunit of the AMPK. AMPK is responsible for regulating fatty acid and cholesterol synthesis. AMPK is activated in response to a wide variety of metabolic stresses and switches off anabolic pathways when ATP levels are depleted.
Alternate Names
5'-AMP-activated protein kinase catalytic subunit alpha-1; 5'-AMP-activated protein kinase, catalytic alpha-1 chain; ACACA kinase; acetyl-CoA carboxylase kinase; AMP -activate kinase alpha 1 subunit; AMP-activated protein kinase, catalytic, alpha-1; AMPK; AMPK alpha 1; AMPK subunit alpha-1; AMPKa1; HMGCR kinase; hydroxymethylglutaryl-CoA reductase kinase; protein kinase, AMP-activated, alpha 1 catalytic subunit; tau-protein kinase PRKAA1
Applications
All western blot analysis is performed using 5% Milk-TBST for blocking and as antibody diluent. Primary antibody is incubated overnight.
Western blots of cell lysates are performed using Goat anti-Rabbit IgG Heavy and Light Chain Antibody (Cat. No. A120-101P).
Western blots of immunoprecipitates are performed using Goat anti-Rabbit Light Chain HRP Conjugate (Cat. No. A120-113P) with 5% Normal Pig Serum (Cat. No. S100-020) added to the blocking buffer.