Rabbit anti-ANAPC10/APC10 Antibody Affinity Purified
Product Details
Specifications
The epitope recognized by A304-981A maps to a region between residue 135 to 185 of human Anaphase-promoting complex subunit 10 using the numbering given in entry Q9UM13.1 (GeneID 10393).
Immunoglobulin concentration was determined using Beer’s Law where 1mg/mL IgG has an A280 of 1.4. Antibody was affinity purified using an epitope specific to ANAPC10/APC10 immobilized on solid support.
The epitope recognized by A304-981A-T maps to a region between residue 135 to 185 of human Anaphase-promoting complex subunit 10 using the numbering given in entry Q9UM13.1 (GeneID 10393).
Immunoglobulin concentration was determined using Beer’s Law where 1mg/mL IgG has an A280 of 1.4.
Additional Product Information
ANAPC10/APC10 is a core subunit of the anaphase-promoting complex (APC), or cyclosome, a ubiquitin protein ligase that is essential for progression through the cell cycle. APC initiates sister chromatid separation by ubiquitinating the anaphase inhibitor securin (PTTG1; MIM 604147) and triggers exit from mitosis by ubiquitinating cyclin B (CCNB1; MIM 123836), the activating subunit of cyclin-dependent kinase-1 (CDK1; MIM 116940) (summary by Wendt et al., 2001 [PubMed 11524682]) [taken from NCBI Entrez Gene (Gene ID: 10393)].
Alternate Names
anaphase-promoting complex subunit 10; APC10; cyclosome subunit 10; DOC1
Applications
All western blot analysis is performed using 5% Milk-TBST for blocking and as antibody diluent. Primary antibody is incubated overnight.
Western blots of immunoprecipitates are performed using Goat anti-Rabbit Light Chain HRP Conjugate (Cat. No. A120-113P) with 5% Normal Pig Serum (Cat. No. S100-020) added to the blocking buffer.