Rabbit anti-ANKHD1 Antibody Affinity Purified
Product Details
Specifications
The epitope recognized by A303-306A maps to a region between residue 25 and 75 of human Ankyrin Repeat and KH Domain Containing 1 using the numbering given in entry NP_060217.1 (GeneID 54882).
Immunoglobulin concentration was determined using Beer’s Law where 1mg/mL IgG has an A280 of 1.4. Antibody was affinity purified using an epitope specific to ANKHD1 immobilized on solid support.
The epitope recognized by A303-306A-T maps to a region between residue 25 and 75 of human Ankyrin Repeat and KH Domain Containing 1 using the numbering given in entry NP_060217.1 (GeneID 54882).
Additional Product Information
Ankyrin repeat and KH domain containing 1 (ANKHD1) is also known as multiple ankyrin repeats single KH domain (MASK). ANKDH1 is thought to function as a scaffolding protein, and it may be involved in the regulation of caspases and thereby play an antiapoptotic role in cell survival [taken from NCBI Entrez Gene (Gene ID: 54882)].
Alternate Names
ankyrin repeat and KH domain-containing protein 1; HIV-1 Vpr-binding ankyrin repeat protein; hMASK; MASK; MASK1; Multiple ankyrin repeats single KH domain; multiple ankyrin repeats, single KH-domain homolog; PP2500; VBARP
Applications
All western blot analysis is performed using 5% Milk-TBST for blocking and as antibody diluent. Primary antibody is incubated overnight.
Western blots of cell lysates are performed using Goat anti-Rabbit IgG Heavy and Light Chain Antibody (Cat. No. A120-101P).
Western blots of immunoprecipitates are performed using Goat anti-Rabbit Light Chain HRP Conjugate (Cat. No. A120-113P) with 5% Normal Pig Serum (Cat. No. S100-020) added to the blocking buffer.