Rabbit anti-APC Antibody Affinity Purified
Product Details
Specifications
The epitope recognized by A300-980A-T maps to a region between residue 75 and 125 of human adenomatosis polyposis coli using the numbering given in entry NP_000029.2 (GeneID 324).
Immunoglobulin concentration was determined using Beer’s Law where 1mg/mL IgG has an A280 of 1.4. Antibody was affinity purified using an epitope specific to APC immobilized on solid support.
The epitope recognized by A300-980A maps to a region between residue 75 and 125 of human adenomatosis polyposis coli using the numbering given in entry NP_000029.2 (GeneID 324).
Immunoglobulin concentration was determined using Beer’s Law where 1mg/mL IgG has an A280 of 1.4.
Additional Product Information
Adenomatosis polyposis coli (APC) is a tumor suppressor gene found to be mutated in most colon cancers and is the cause of familial adenomatous polyposis (FAP), an autosomal dominant disease that results in a predisposition to malignant colon cancer. APC has been shown to be involved in cell differentiation, migration, proliferation and adhesion. One function of APC involves its role as a scaffolding protein that regulates the phosphorylation and degradation of beta-catenin, a transcriptional regulator in the WNT signaling pathway. Additionally, APC has been found to be important in the regulation of microtubule arrays and mitotic spindle formation.
Alternate Names
adenomatosis polyposis coli tumor suppressor; adenomatous polyposis coli (APC); adenomatous polyposis coli protein; BTPS2; deleted in polyposis 2.5; DP2; DP2.5; DP3; epididymis secretory sperm binding protein; GS; PPP1R46; Protein APC; protein phosphatase 1, regulatory subunit 46; truncated adenomatosis polyposis coli; WNT signaling pathway regulator
Applications
All western blot analysis is performed using 5% Milk-TBST for blocking and as antibody diluent. Primary antibody is incubated overnight.
Western blots of immunoprecipitates are performed using Goat anti-Rabbit Light Chain HRP Conjugate (Cat. No. A120-113P) with 5% Normal Pig Serum (Cat. No. S100-020) added to the blocking buffer.