Rabbit anti-APC4 Antibody Affinity Purified
Product Details
Specifications
The epitope recognized by A301-176A maps to a region between residue 758 and 808 of human anaphase-promoting complex subunit 4 using the numbering given in entry NP_037499.2 (GeneID 29945).
Immunoglobulin concentration was determined using Beer’s Law where 1mg/mL IgG has an A280 of 1.4. Antibody was affinity purified using an epitope specific to APC4 immobilized on solid support.
The epitope recognized by A301-176A-T maps to a region between residue 758 and 808 of human anaphase-promoting complex subunit 4 using the numbering given in entry NP_037499.2 (GeneID 29945).
Additional Product Information
APC4 is a component of the anaphase-promoting complex (APC). The APC complex functions as an ubiquitin ligase that controls cell cycle progression and promotes the transition from metaphase to anaphase. The APC complex is composed of at least 11 APC components. The exact function of APC4 has not been determined.
Alternate Names
anaphase-promoting complex subunit 4; APC4; cyclosome subunit 4
Applications
All western blot analysis is performed using 5% Milk-TBST for blocking and as antibody diluent. Primary antibody is incubated overnight.
Western blots of cell lysates are performed using Goat anti-Rabbit IgG Heavy and Light Chain Antibody (Cat. No. A120-101P).
Western blots of immunoprecipitates are performed using Goat anti-Rabbit Light Chain HRP Conjugate (Cat. No. A120-113P) with 5% Normal Pig Serum (Cat. No. S100-020) added to the blocking buffer.