Rabbit anti-APC7 Antibody Affinity Purified
Product Details
Specifications
The epitope recognized by A302-551A maps to a region between residue 515 and 565 of human anaphase promoting complex subunit 7 using the numbering given in entry AAI41849.1 (GeneID 51434).
Immunoglobulin concentration was determined using Beer’s Law where 1mg/mL IgG has an A280 of 1.4. Antibody was affinity purified using an epitope specific to APC7 immobilized on solid support.
The epitope recognized by A302-551A-T maps to a region between residue 515 and 565 of human anaphase promoting complex subunit 7 using the numbering given in entry AAI41849.1 (GeneID 51434).
Additional Product Information
Anaphase promoting complex subunit 7 (APC7) is a component of the APC/c (anaphase promoting complex/cyclosome) which is responsible for the ubiquitination and degradation of securin and cyclin B which prompts the onset of anaphase and exit from mitosis. The APC/c is composed of at least 11 subunits.
Alternate Names
anaphase-promoting complex subunit 7; APC7; cyclosome subunit 7
Applications
All western blot analysis is performed using 5% Milk-TBST for blocking and as antibody diluent. Primary antibody is incubated overnight.
Western blots of cell lysates are performed using Goat anti-Rabbit IgG Heavy and Light Chain Antibody (Cat. No. A120-101P).
Western blots of immunoprecipitates are performed using Goat anti-Rabbit Light Chain HRP Conjugate (Cat. No. A120-113P) with 5% Normal Pig Serum (Cat. No. S100-020) added to the blocking buffer.