Rabbit anti-ARHGAP5 Antibody Affinity Purified
Product Details
Specifications
The epitope recognized by A304-548A maps to a region between residue 1050 to 1100 of human Rho GTPase Activating Protein 5 using the numbering given in entry NP_001025226.1 (GeneID 394).
Immunoglobulin concentration was determined using Beer’s Law where 1mg/mL IgG has an A280 of 1.4. Antibody was affinity purified using an epitope specific to ARHGAP5 immobilized on solid support.
The epitope recognized by A304-548A-T maps to a region between residue 1050 to 1100 of human Rho GTPase Activating Protein 5 using the numbering given in entry NP_001025226.1 (GeneID 394).
Additional Product Information
ARHGAP5 is a GTPase-activating protein for Rho family members. May play a role in the reduction of the p21rasGTPase-activating potential of RASA1/p120GAP [taken from the Universal Protein Resource (UniProt) www.uniprot.org/uniprot/Q13017].
Alternate Names
GFI2; growth factor independent 2; p100 RasGAP-associated p105 protein; p105 RhoGAP; p190-B; p190BRhoGAP; rho GTPase-activating protein 5; RhoGAP5; rho-type GTPase-activating protein 5
Applications
All western blot analysis is performed using 5% Milk-TBST for blocking and as antibody diluent. Primary antibody is incubated overnight.
Western blots of cell lysates are performed using Goat anti-Rabbit IgG Heavy and Light Chain Antibody (Cat. No. A120-101P).
Western blots of immunoprecipitates are performed using Goat anti-Rabbit Light Chain HRP Conjugate (Cat. No. A120-113P) with 5% Normal Pig Serum (Cat. No. S100-020) added to the blocking buffer.