Rabbit anti-ARID1A/BAF250 Recombinant Monoclonal Antibody [BLR279L]
Product Details
Specifications
Request Formulation Change Borate Buffered Saline (BBS) pH 8.2 with 0.09% Sodium Azide, BSA-Free
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Immunogen was a peptide representing a region between residue 750 and 800 of human AT-rich interactive domain-containing protein 1A using the numbering given in entry NP_006006.3 (Gene ID 8289).
Additional Product Information
The SWI/SNF-related, matrix-associated, actin-dependent regulators of chromatin (SMARC), also called BRG1-associated factors (BAFs), have been identified as components of the human SWI/SNF-like chromatin-remodeling protein complexes. SWI/SNF complexes possess ATPase and DNA helicase activity that promotes transcriptional activation of genes normally repressed by chromatin structure. ARID1A/BAF250 is a noncatalytic subunit that is a member of the ARID (AT-rich interactive domain-containing protein) family of DNA binding proteins. ARID1A/BAF250 has been shown to play a role in the tumor suppressor activity of the SWI/SNF complexes. ARID1A/BAF250 is required for differentiation-associated cell cycle arrest.
Alternate Names
ARID domain-containing protein 1A;AT rich interactive domain 1A (SWI-like);AT-rich interactive domain-containing protein 1A;B120;BAF250;BAF250a;BM029;brain protein 120;BRG1-associated factor 250;BRG1-associated factor 250a;C1orf4;chromatin remodeling factor p250;CSS2;ELD;hELD;hOSA1;MRD14;osa homolog 1;OSA1;OSA1 nuclear protein;P270;SMARCF1;SWI/SNF complex protein p270;SWI/SNF-related, matrix-associated, actin-dependent regulator of chromatin subfamily F member 1;SWI-like protein
Applications
All western blot analysis is performed using 5% Milk-TBST for blocking and as antibody diluent. Primary antibody is incubated overnight.
Western blots of cell lysates are performed using Goat anti-Rabbit IgG Heavy and Light Chain Antibody (Cat. No. A120-101P).
Western blots of immunoprecipitates are performed using Goat anti-Rabbit Light Chain HRP Conjugate (Cat. No. A120-113P) with 5% Normal Pig Serum (Cat. No. S100-020) added to the blocking buffer.