Rabbit anti-ARID4B Antibody Affinity Purified
Product Details
Specifications
The epitope recognized by A302-233A maps to a region between residue 125 and 175 of human AT rich interactive domain 4B using the numbering given in entry NP_057458.4 (GeneID 51742).
Immunoglobulin concentration was determined using Beer’s Law where 1mg/mL IgG has an A280 of 1.4. Antibody was affinity purified using an epitope specific to ARID4B immobilized on solid support.
The epitope recognized by A302-233A-T maps to a region between residue 125 and 175 of human AT rich interactive domain 4B using the numbering given in entry NP_057458.4 (GeneID 51742).
Additional Product Information
ARID4B (AT-rich interactive domain-containing protein 4B) is also known as RbBP1L1 (retinoblastoma binding protein 1-like 1) and is a retinoblastoma binding protein and member of the AT-rich interaction domain (ARID) family of proteins. In addition to the ARID DNA binding domain, ARID4B also contains a chromo domain and a TUDOR domain, indicating a function in chromatin organization and protein-protein interactions with methylated protein substrates. As a subunit of the SIN3A transcriptional co-repressor, ARID4B is implicated to be involved in chromatin remodeling and epigenetic modifications. ARID4B may play a role in the pathogenesis of breast cancer and other malignancies.
Alternate Names
180 kDa Sin3-associated polypeptide; ARID domain-containing protein 4B; AT rich interactive domain 4B (RBP1-like); AT-rich interactive domain-containing protein 4B; BCAA; BRCAA1; breast cancer-associated antigen 1; breast cancer-associated antigen BRCAA1; breast carcinoma-associated antigen; histone deacetylase complex subunit SAP180; Rb-binding protein homolog; RBBP1L1; RBP1L1; retinoblastoma-binding protein 1-like 1; SAP180; SIN3A-associated protein 180; sin3-associated polypeptide p180
Applications
All western blot analysis is performed using 5% Milk-TBST for blocking and as antibody diluent. Primary antibody is incubated overnight.
Western blots of cell lysates are performed using Goat anti-Rabbit IgG Heavy and Light Chain Antibody (Cat. No. A120-101P).
Western blots of immunoprecipitates are performed using Goat anti-Rabbit Light Chain HRP Conjugate (Cat. No. A120-113P) with 5% Normal Pig Serum (Cat. No. S100-020) added to the blocking buffer.