Rabbit anti-ATF1 Antibody Affinity Purified
Product Details
Specifications
The epitope recognized by A303-034A maps to a region between residue 1 and 50 of human Activating Transcription Factor 1 using the numbering given in entry NP_005162.1 (GeneID 466).
Immunoglobulin concentration was determined using Beer’s Law where 1mg/mL IgG has an A280 of 1.4. Antibody was affinity purified using an epitope specific to ATF1 immobilized on solid support.
The epitope recognized by A303-034A-T maps to a region between residue 1 and 50 of human Activating Transcription Factor 1 using the numbering given in entry NP_005162.1 (GeneID 466).
Additional Product Information
ATF1 (activating transcription factor 1) is a transcription factor that binds the cAMP response element (CRE) (consensus: 5'-GTGACGT[AC][AG]-3'), a sequence present in many viral and cellular promoters.
Alternate Names
Activating transcription factor 1; cAMP-dependent transcription factor ATF-1; cyclic AMP-dependent transcription factor ATF-1; EWS-ATF1; FUS/ATF-1; Protein TREB36; TREB36
Applications
All western blot analysis is performed using 5% Milk-TBST for blocking and as antibody diluent. Primary antibody is incubated overnight.
Western blots of cell lysates are performed using Goat anti-Rabbit IgG Heavy and Light Chain Antibody (Cat. No. A120-101P).
Western blots of immunoprecipitates are performed using Goat anti-Rabbit Light Chain HRP Conjugate (Cat. No. A120-113P) with 5% Normal Pig Serum (Cat. No. S100-020) added to the blocking buffer.