Rabbit anti-Atg16L1 Antibody Affinity Purified
Product Details
Specifications
The epitope recognized by A303-294A maps to a region between residue 50 and 100 of human ATG16 Autophagy Related 16-like 1 using the numbering given in entry NP_110430.5 (GeneID 55054).
Immunoglobulin concentration was determined using Beer’s Law where 1mg/mL IgG has an A280 of 1.4. Antibody was affinity purified using an epitope specific to Atg16L1 immobilized on solid support.
The epitope recognized by A303-294A-T maps to a region between residue 50 and 100 of human ATG16 Autophagy Related 16-like 1 using the numbering given in entry NP_110430.5 (GeneID 55054).
Additional Product Information
ATG16 autophagy related 16-like (ATG16L1) is part of a large protein complex that is necessary for autophagy, the major process by which intracellular components are targeted to lysosomes for degradation. Defects in the ATG16L1 gene are a cause of susceptibility to inflammatory bowel disease type 10 (IBD10) [taken from NCBI Entrez Gene (Gene ID: 55054)].
Alternate Names
APG16L; APG16L beta; APG16-like 1; ATG16 autophagy related 16-like 1; ATG16A; ATG16L; autophagy-related protein 16-1; IBD10; WD repeat domain 30; WDR30
Applications
All western blot analysis is performed using 5% Milk-TBST for blocking and as antibody diluent. Primary antibody is incubated overnight.
Western blots of cell lysates are performed using Goat anti-Rabbit IgG Heavy and Light Chain Antibody (Cat. No. A120-101P).
Western blots of immunoprecipitates are performed using Goat anti-Rabbit Light Chain HRP Conjugate (Cat. No. A120-113P) with 5% Normal Pig Serum (Cat. No. S100-020) added to the blocking buffer.