Rabbit anti-Atherin Antibody Affinity Purified
Product Details
Specifications
The epitope recognized by A303-578A maps to a region between residue 488 and 538 of human Sterile Alpha Motif Domain Containing 1 using the numbering given in entry NP_612361.1 (GeneID 90378).
Immunoglobulin concentration was determined using Beer’s Law where 1mg/mL IgG has an A280 of 1.4. Antibody was affinity purified using an epitope specific to Atherin immobilized on solid support.
The epitope recognized by A303-578A-T maps to a region between residue 488 and 538 of human Sterile Alpha Motif Domain Containing 1 using the numbering given in entry NP_612361.1 (GeneID 90378).
Additional Product Information
Atherin is encoded by the SAMD1 gene (sterile alpha motif-domain-containing protein 1) and is suspected to play a role in atherogenesis. Atherin 1 is a cytoplasmic and secreted protein expressed in atherosclerotic lesions.
Alternate Names
atherin; SAM domain containing 1; SAM domain-containing protein 1; sterile alpha motif domain-containing protein 1
Applications
All western blot analysis is performed using 5% Milk-TBST for blocking and as antibody diluent. Primary antibody is incubated overnight.
Western blots of cell lysates are performed using Goat anti-Rabbit IgG Heavy and Light Chain Antibody (Cat. No. A120-101P).
Western blots of immunoprecipitates are performed using Goat anti-Rabbit Light Chain HRP Conjugate (Cat. No. A120-113P) with 5% Normal Pig Serum (Cat. No. S100-020) added to the blocking buffer.