Rabbit anti-ATP6V1H Antibody Affinity Purified
Product Details
Specifications
The epitope recognized by A304-308A-T maps to a region between residue 400 to 450 of human ATPase, H+ Transporting, Lysosomal 50/57kDa, V1 Subunit H using the numbering given in entry NP_057025.2 (GeneID 51606).
Immunoglobulin concentration was determined using Beer’s Law where 1mg/mL IgG has an A280 of 1.4. Antibody was affinity purified using an epitope specific to ATP6V1H immobilized on solid support.
The epitope recognized by A304-308A maps to a region between residue 400 to 450 of human ATPase, H+ Transporting, Lysosomal 50/57kDa, V1 Subunit H using the numbering given in entry NP_057025.2 (GeneID 51606).
Immunoglobulin concentration was determined using Beer’s Law where 1mg/mL IgG has an A280 of 1.4.
Additional Product Information
V-type proton ATPase subunit H (ATP6V1H) is a subunit of the peripheral V1 complex of vacuolar ATPase. Subunit H activates the ATPase activity of the enzyme and couples ATPase activity to proton flow. Vacuolar ATPase is responsible for acidifying a variety of intracellular compartments in eukaryotic cells, thus providing most of the energy required for transport processes in the vacuolar system (By similarity). ATP6V1H is involved in the endocytosis mediated by clathrin-coated pits, required for the formation of endosomes [taken from the Universal Protein Resource (UniProt) www.uniprot.org/uniprot/Q9UI12].
Alternate Names
ATPase, H+ transporting, lysosomal 50/57kDa, V1 subunit H; CGI-11; MSTP042; NBP1; nef-binding protein 1; protein VMA13 homolog; SFD; SFDalpha; SFDbeta; vacuolar ATP synthase subunit H; vacuolar ATPase subunit H; vacuolar proton pump subunit H; vacuolar proton pump subunit SFD; V-ATPase 50/57 kDa subunits; V-ATPase subunit H; VMA13; V-type proton ATPase subunit H
Applications
All western blot analysis is performed using 5% Milk-TBST for blocking and as antibody diluent. Primary antibody is incubated overnight.
Western blots of cell lysates are performed using Goat anti-Rabbit IgG Heavy and Light Chain Antibody (Cat. No. A120-101P).
Western blots of immunoprecipitates are performed using Goat anti-Rabbit Light Chain HRP Conjugate (Cat. No. A120-113P) with 5% Normal Pig Serum (Cat. No. S100-020) added to the blocking buffer.