Rabbit anti-Aurora A Antibody Affinity Purified
Product Details
Specifications
The epitope recognized by A300-071A maps to a region between residues 75 and 125 of human Aurora A Kinase using the numbering given in entry NP_003591.2 (GeneID 6790).
Immunoglobulin concentration was determined using Beer’s Law where 1mg/mL IgG has an A280 of 1.4. Antibody was affinity purified using an epitope specific to Aurora A immobilized on solid support.
The epitope recognized by A300-071A-T maps to a region between residues 75 and 125 of human Aurora A Kinase using the numbering given in entry NP_003591.2 (GeneID 6790).
Immunoglobulin concentration was determined using Beer’s Law where 1mg/mL IgG has an A280 of 1.4.
Additional Product Information
Aurora A is a serine-threonine protein kinase that localizes to centrosomes and spindle poles and is important to spindle assembly and chromosome congression. Substrates of Aurora A kinase activity include BRCA1, CDC25B, and NDEL1. Aurora A has been found to be overexpressed in human cancers.
Alternate Names
AIK; ARK1; ARK-1; AURA; aurora 2; aurora kinase A; aurora/IPL1-like kinase; aurora/IPL1-related kinase 1; Aurora-related kinase 1; breast tumor-amplified kinase; BTAK; hARK1; PPP1R47; protein phosphatase 1, regulatory subunit 47; serine/threonine protein kinase 15; Serine/threonine-protein kinase 15; serine/threonine-protein kinase 6; serine/threonine-protein kinase aurora-A; STK15; STK6; STK7
Applications
All western blot analysis is performed using 5% Milk-TBST for blocking and as antibody diluent. Primary antibody is incubated overnight.
Western blots of cell lysates are performed using Goat anti-Rabbit IgG Heavy and Light Chain Antibody (Cat. No. A120-101P).
Western blots of immunoprecipitates are performed using Goat anti-Rabbit Light Chain HRP Conjugate (Cat. No. A120-113P) with 5% Normal Pig Serum (Cat. No. S100-020) added to the blocking buffer.