Rabbit anti-B7-H3/CD276 Antibody Affinity Purified
Product Details
Specifications
The epitope recognized by A304-368A maps to a region between residue 484 to 534 of human CD276 Molecule using the numbering given in entry NP_001019907.1 (GeneID 80381).
Immunoglobulin concentration was determined using Beer’s Law where 1mg/mL IgG has an A280 of 1.4. Antibody was affinity purified using an epitope specific to B7-H3/CD276 immobilized on solid support.
The epitope recognized by A304-368A-T maps to a region between residue 484 to 534 of human CD276 Molecule using the numbering given in entry NP_001019907.1 (GeneID 80381).
Immunoglobulin concentration was determined using Beer’s Law where 1mg/mL IgG has an A280 of 1.4.
Additional Product Information
CD276 may participate in the regulation of T-cell-mediated immune response. CD276 may play a protective role in tumor cells by inhibiting natural-killer mediated cell lysis as well as a role of marker for detection of neuroblastoma cells. It may also be involved in the development of acute and chronic transplant rejection and in the regulation of lymphocytic activity at mucosal surfaces and could also play a key role in providing the placenta and fetus with a suitable immunological environment throughout pregnancy [taken from the Universal Protein Resource (UniProt) www.uniprot.org/uniprot/Q5ZPR3].
Alternate Names
4Ig-B7-H3; B7 homolog 3; B7H3; B7-H3; B7RP-2; CD antigen CD276; CD276 antigen; costimulatory molecule
Applications
All western blot analysis is performed using 5% Milk-TBST for blocking and as antibody diluent. Primary antibody is incubated overnight.
Western blots of immunoprecipitates are performed using Goat anti-Rabbit Light Chain HRP Conjugate (Cat. No. A120-113P) with 5% Normal Pig Serum (Cat. No. S100-020) added to the blocking buffer.