Rabbit anti-BCAR1/p130CAS Antibody Affinity Purified
Product Details
Specifications
The epitope recognized by A301-667A maps to a region between residue 275 and 325 of human breast cancer anti-estrogen resistance 1 (Crk-associated substrate p130Cas) using the numbering given in entry NP_055382.2 (GeneID 9564).
Immunoglobulin concentration was determined using Beer’s Law where 1mg/mL IgG has an A280 of 1.4. Antibody was affinity purified using an epitope specific to BCAR1/p130CAS immobilized on solid support.
The epitope recognized by A301-667A-T maps to a region between residue 275 and 325 of human breast cancer anti-estrogen resistance 1 (Crk-associated substrate p130Cas) using the numbering given in entry NP_055382.2 (GeneID 9564).
Additional Product Information
BCAR1/p130CAS functions as a scaffolding protein for components of various kinase signaling pathways. It is involved in integrin signaling for cell motility as well as netrin signaling for the guidance of commissural axons. Overexpression of BCAR1/p130CAS has been shown to confer antiestrogen resistance in breast cancer cells.
Alternate Names
BCAR1, Cas family scaffolding protein; breast cancer anti-estrogen resistance protein 1; CAS; Cas scaffolding protein family member 1; CAS1; CASS1; CRKAS; CRK-associated substrate; Crk-associated substrate p130Cas; P130Cas
Applications
All western blot analysis is performed using 5% Milk-TBST for blocking and as antibody diluent. Primary antibody is incubated overnight.
Western blots of cell lysates are performed using Goat anti-Rabbit IgG Heavy and Light Chain Antibody (Cat. No. A120-101P).
Western blots of immunoprecipitates are performed using Goat anti-Rabbit Light Chain HRP Conjugate (Cat. No. A120-113P) with 5% Normal Pig Serum (Cat. No. S100-020) added to the blocking buffer.