Rabbit anti-beta Catenin Antibody Affinity Purified
Product Details
Specifications
The epitope recognized by A302-011A maps to a region between residue 710 and 760 of human beta-catenin using the numbering given in entry NP_001895.1 (GeneID 1499).
Immunoglobulin concentration was determined using Beer’s Law where 1mg/mL IgG has an A280 of 1.4. Antibody was affinity purified using an epitope specific to beta Catenin immobilized on solid support.
The epitope recognized by A302-011A-T maps to a region between residue 710 and 760 of human beta-catenin using the numbering given in entry NP_001895.1 (GeneID 1499).
Additional Product Information
beta Catenin plays an integral role in the Wnt signaling pathway and is critical to morphogenic signaling and body axis specification. Beta Catenin exists in complexes with cadherins, transmembrane proteins that mediate cell adhesion. When Wnt-signaling proteins bind their receptors, beta Catenin is stabilized and accumulates in the cell. Translocation of stabilized beta Catenin allows its association with transcription factors and the activation of specific gene expression.
Alternate Names
armadillo; Beta-catenin; catenin (cadherin-associated protein), beta 1, 88kDa; catenin beta-1; CTNNB; EVR7; MRD19
Applications
All western blot analysis is performed using 5% Milk-TBST for blocking and as antibody diluent. Primary antibody is incubated overnight.
Western blots of cell lysates are performed using Goat anti-Rabbit IgG Heavy and Light Chain Antibody (Cat. No. A120-101P).
Western blots of immunoprecipitates are performed using Goat anti-Rabbit Light Chain HRP Conjugate (Cat. No. A120-113P) with 5% Normal Pig Serum (Cat. No. S100-020) added to the blocking buffer.