Rabbit anti-BHC110/LSD1 Antibody Affinity Purified
Product Details
Specifications
The epitope recognized by A300-216A maps to a region between residues 825 and the C-terminus (residue 886) of human FAD-binding protein BRAF35-HDAC complex 110 kDa (lysine-specific histone demethylase 1) using the numbering given in TrEMBL entry O60341 (GeneID 23028).
Immunoglobulin concentration was determined using Beer’s Law where 1mg/mL IgG has an A280 of 1.4. Antibody was affinity purified using an epitope specific to BHC110/LSD1 immobilized on solid support.
The epitope recognized by A300-216A-T maps to a region between residues 825 and the C-terminus (residue 886) of human FAD-binding protein BRAF35-HDAC complex 110 kDa (lysine-specific histone demethylase 1) using the numbering given in TrEMBL entry O60341 (GeneID 23028).
Immunoglobulin concentration was determined using Beer’s Law where 1mg/mL IgG has an A280 of 1.4.
Additional Product Information
BHC110/LSD1 is a histone demethylase and a component of several histone deacetylase complexes. BHC110/LSD1 is able to specifically catalyze the demethylation of Lys4 of histone H3. As part of histone deacetylase complexes BHC110/LSD1 functions as a corepressor of transcription. BHC110/LSD1 has also been demonstrated to interact with the androgen receptor and stimulate androgen-receptor-dependent transcription.
Alternate Names
amine oxidase (flavin containing) domain 2; AOF2; BHC110; BRAF35-HDAC complex protein BHC110; CPRF; FAD-binding protein BRAF35-HDAC complex, 110 kDa subunit; flavin-containing amine oxidase domain-containing protein 2; KDM1; LSD1; lysine (K)-specific demethylase 1A; lysine-specific histone demethylase 1; lysine-specific histone demethylase 1A
Applications
All western blot analysis is performed using 5% Milk-TBST for blocking and as antibody diluent. Primary antibody is incubated overnight.
Western blots of cell lysates are performed using Goat anti-Rabbit IgG Heavy and Light Chain Antibody (Cat. No. A120-101P).