Rabbit anti-BLAP75 Antibody Affinity Purified
Product Details
Specifications
The epitope recognized by A300-631A-T maps to a region between residues 275 and 300 of human BLM Associated Protein, 75kDA using the numbering given in entry NP_079221.1 (GeneID 80010). Antibody was affinity purified using an epitope specific to BLAP75 immobilized on solid support.
The epitope recognized by A300-631A maps to a region between residues 275 and 300 of human BLM Associated Protein, 75kDA using the numbering given in entry NP_079221.1 (GeneID 80010).
Immunoglobulin concentration was determined using Beer’s Law where 1mg/mL IgG has an A280 of 1.4.
Additional Product Information
BLM-associated polypeptide, 75 kDa (BLAP75) is a component of a dissolvasome complex that contains BLM (Bloom syndrome protein) and TopoIII-alpha and functions to dissolve double Holliday junctions and suppress DNA crossover formation in mitotic cells.
Alternate Names
BLAP75; BLM-associated polypeptide, 75 kDa; BLM-associated protein 75 kDa; BLM-associated protein of 75 kDa; C9orf76; FAAP75; homolog of yeast RecQ-mediated genome instability 1 (RMI1); recQ-mediated genome instability protein 1; RMI1, RecQ mediated genome instability 1, homolog
Applications
All western blot analysis is performed using 5% Milk-TBST for blocking and as antibody diluent. Primary antibody is incubated overnight.
Western blots of cell lysates are performed using Goat anti-Rabbit IgG Heavy and Light Chain Antibody (Cat. No. A120-101P).
Western blots of immunoprecipitates are performed using Goat anti-Rabbit Light Chain HRP Conjugate (Cat. No. A120-113P) with 5% Normal Pig Serum (Cat. No. S100-020) added to the blocking buffer.