Rabbit anti-BMAL1 Antibody Affinity Purified
Product Details
Specifications
The epitope recognized by A302-616A maps to a region between residue 575 and 625 of human brain and muscle Arnt-like protein-1 using the numbering given in entry NP_001025443.1 (GeneID 406).
Immunoglobulin concentration was determined using Beer’s Law where 1mg/mL IgG has an A280 of 1.4. Antibody was affinity purified using an epitope specific to BMAL1 immobilized on solid support.
The epitope recognized by A302-616A-T maps to a region between residue 575 and 625 of human brain and muscle Arnt-like protein-1 using the numbering given in entry NP_001025443.1 (GeneID 406).
Additional Product Information
BMAL1 [brain and muscle aryl hydrocarbon receptor nuclear translocator (ARNT)-like 1] is a basic helix-loop-helix protein that forms a heterodimer with CLOCK. This complex binds an E-box upstream of the PER1 gene, activating this gene and possibly other circadian rhythym-associated genes [taken from NCBI Entrez Gene (GeneID: 406)].
Alternate Names
ARNT-like protein 1, brain and muscle; aryl hydrocarbon receptor nuclear translocator-like protein 1; basic helix-loop-helix family member e5; basic-helix-loop-helix-PAS orphan MOP3; basic-helix-loop-helix-PAS protein MOP3; bHLHe5; bHLH-PAS protein JAP3; BMAL1; BMAL1c; brain and muscle ARNT-like 1; class E basic helix-loop-helix protein 5; JAP3; member of PAS protein 3; member of PAS superfamily 3; MOP3; PAS domain containing 3; PAS domain-containing protein 3; PASD3; testis tissue sperm-binding protein Li 50e; TIC
Applications
All western blot analysis is performed using 5% Milk-TBST for blocking and as antibody diluent. Primary antibody is incubated overnight.
Western blots of cell lysates are performed using Goat anti-Rabbit IgG Heavy and Light Chain Antibody (Cat. No. A120-101P).
Western blots of immunoprecipitates are performed using Goat anti-Rabbit Light Chain HRP Conjugate (Cat. No. A120-113P) with 5% Normal Pig Serum (Cat. No. S100-020) added to the blocking buffer.