Rabbit anti-BOP1 Antibody Affinity Purified
Product Details
Specifications
The epitope recognized by A302-148A-T maps to a region between residue 300 and 350 of human block of proliferation 1 using the numbering given in entry NP_056016.1 (GeneID 23246). Antibody was affinity purified using an epitope specific to BOP1 immobilized on solid support.
The epitope recognized by A302-148A maps to a region between residue 300 and 350 of human block of proliferation 1 using the numbering given in entry NP_056016.1 (GeneID 23246).
Immunoglobulin concentration was determined using Beer’s Law where 1mg/mL IgG has an A280 of 1.4.
Additional Product Information
BOP1 (block of proliferation 1) is a component of the PeBoW (Pes1, Bop, WDR12) complex that plays a role in the processing of 28S and 5.8S ribosomal RNAs and the formation of the 60S ribosome. BOP1 contains seven WD repeats and was originally identified as a cDNA which inhibited cell proliferation. The deregulation of the BOP1 gene is proposed to contribute to colorectal tumorigenesis.
Alternate Names
Block of proliferation 1 protein; ribosome biogenesis protein BOP1
Applications
All western blot analysis is performed using 5% Milk-TBST for blocking and as antibody diluent. Primary antibody is incubated overnight.
Western blots of cell lysates are performed using Goat anti-Rabbit IgG Heavy and Light Chain Antibody (Cat. No. A120-101P).
Western blots of immunoprecipitates are performed using Goat anti-Rabbit Light Chain HRP Conjugate (Cat. No. A120-113P) with 5% Normal Pig Serum (Cat. No. S100-020) added to the blocking buffer.