Rabbit anti-Bovine beta-Lactoglobulin Antibody HRP Conjugated
Product Details
Specifications
Request Formulation Change Phosphate Buffered Saline (PBS) containing 0.09% Sodium Azide
Request Formulation Change Serum containing 0.1% Sodium Azide
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Prior to conjugation, immunoglobulin concentration was determined using Beer’s Law where 1mg/mL IgG has an A280 of 1.4.
Molar enzyme/antibody protein ratio is 4:1.
By immunoelectrophoresis and ELISA this antibody reacts specifically with Bovine beta-Lactoglobulin. This antibody may cross react with beta lactoglobulin from other species. Antiserum was solid phase adsorbed to ensure specificity. The antibody was isolated by affinity chromatography using antigen coupled to agarose beads.
Immunoglobulin concentration was determined using Beer’s Law where 1mg/mL IgG has an A280 of 1.4.
By immunoelectrophoresis and ELISA this antibody reacts specifically with Bovine beta-Lactoglobulin. This antibody may cross react with beta lactoglobulin from other species. By immunoelectrophoresis, the antiserum reacts specifically with Bovine beta-Lactoglobulin. No antibody was detected against other milk proteins.
This antibody may cross react with beta lactoglobulin from other species.
Additional Product Information
Beta-lactoglobulin is a major protein found in dairy products and is not present in human milk. It has been identified as a potential milk allergen, and is responsible for transport of both iron and hydrophobic molecules.
Applications
Not all listed applications have been specifically tested by our laboratory.
For use in precipitin gel reactions (e.g. immunoelectrophoresis (IEP) or double diffusion Ouchterlony (DD)).
Optimal working dilutions should be determined experimentally by the investigator.