Rabbit anti-BRD9 Recombinant Monoclonal Antibody [BLR153J]
Product Details
Specifications
Immunogen was a peptide representing a region between residue 547 and the C-terminus (residue 597) of human Bromodomain-containing protein 9 using the numbering given in entry NP_076413.3 (Gene ID 65980).
Additional Product Information
BRD9 plays a role in chromatin remodeling and regulation of transcription. It acts as a chromatin reader that recognizes and binds acylated histones: binds histones that are acetylated and/or butyrylated. BRD9 is a component of SWI/SNF chromatin remodeling subcomplex GBAF that carries out key enzymatic activities, changing chromatin structure by altering DNA-histone contacts within a nucleosome in an ATP-dependent manner. BRD9 also orchestrates RAD51-RAD54 complex formation and thereby plays a role in homologous recombination [taken from the Universal Protein Resource (UniProt) www.uniprot.org/uniprot/Q9H8M2].
Alternate Names
bromodomain-containing protein 9; LAVS3040; PRO9856; rhabdomyosarcoma antigen MU-RMS-40.8; sarcoma antigen NY-SAR-29
Applications
All western blot analysis is performed using 5% Milk-TBST for blocking and as antibody diluent. Primary antibody is incubated overnight.
Western blots of cell lysates are performed using Goat anti-Rabbit IgG Heavy and Light Chain Antibody (Cat. No. A120-101P).
Western blots of immunoprecipitates are performed using Goat anti-Rabbit Light Chain HRP Conjugate (Cat. No. A120-113P) with 5% Normal Pig Serum (Cat. No. S100-020) added to the blocking buffer.